Development of antisense RNA-mediated quantifiable inhibition for metabolic regulation.

ABSTRACT: Trans-regulating elements such as noncoding RNAs are crucial in modifying cells, and has shown broad application in synthetic biology, metabolic engineering and RNA therapies. Although effective, titration of the regulatory levels of such elements is less explored. Encouraged by the need of fine-tuning cellular functions, we studied key parameters of the antisense RNA design including oligonucleotide length, targeting region and relative dosage to achieve differentiated inhibition. We determined a 30-nucleotide configuration that renders efficient and robust inhibition. We found that by targeting the core RBS region proportionally, quantifiable inhibition levels can be rationally obtained. A mathematic model was established accordingly with refined energy terms and successfully validated b

SUBMITTER: Zhang R 

PROVIDER: S-EPMC7921874 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature