Proteomic profiling of commercial dust mite skin prick test solutions and allergy vaccines from India.

ABSTRACT:

Background

Skin prick test (SPT) solutions and allergy vaccines (AVs) are crucial tools for diagnosis and therapy of allergies. It was the aim of this study to corroborate the content of products for diagnosis and treatment of dust mite allergies that are produced and sold in India.

Methods

SDS-PAGE, immunoblots and high-resolution mass spectrometric analysis was performed with 16 house dust mite (HDM) SPT solutions and AVs from 3 Indian manufacturers. Authority-approved European SPT solutions and in-house extracts were used as references.

Results

From the 5 Indian Dermatophagoides pteronyssinus products, none contained proteins from this source. Instead, 1 sample contained Dermatophagoides farinae and human serum proteins, 4 products contained allergens

SUBMITTER: Huber S 

PROVIDER: S-EPMC7921882 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature