Epigenetic effects of Bisphenol A on granulosa cells of mouse follicles during in vitro culture: An experimental study.

ABSTRACT:

Background

Bisphenol A (BPA), a synthetic endocrine-disrupting chemical, is a reproductive toxicant. Granulosa cells have significant roles in follicle development, and KIT ligand (KITL) and Anti-Müllerian hormone (AMH) are essential biomolecules produced by them during folliculogenesis.

Objective

Due to the widespread use of BPA and its potential epigenetic effects, this study examined the impact of BPA on promoter methylation of amh and kitl genes in mouse granulosa cells.

Materials and methods

Preantral follicles were isolated from ovaries of immature mice and cultured for eight days. Then, follicles were treated with 50 and 100 μM of BPA, and 0.01% (v/v) ethanol for 24 and 72 hr. Growth and degeneration of follicles and antrum formation were analyzed

SUBMITTER: Khaghani AJ 

PROVIDER: S-EPMC7922291 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature