CPSF6 links alternative polyadenylation to metabolism adaption in hepatocellular carcinoma progression.

ABSTRACT:

Background

Alternative polyadenylation (APA) is an important mechanism of gene expression regulation through generation of RNA isoforms with distinct 3' termini. Increasing evidence has revealed that APA is actively involved in development and disease, including hepatocellular carcinoma (HCC). However, how APA functions in tumor formation and progression remains elusive. In this study, we investigated the role of cleavage factor I (CFIm) subunit CPSF6 in human hepatocellular carcinoma (HCC).

Methods

Expression levels of CPSF6 in clinical tissues and cell lines were determined by qRT-PCR and western blot. Functional assays, including the cell number, MTT, colony formation and transwell, were used to determine the oncogenic role of CPSF6 in HCC. Animal experiments were used to

SUBMITTER: Tan S 

PROVIDER: S-EPMC7923339 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature