Adaptation of Oxford Nanopore technology for hepatitis C whole genome sequencing and identification of within-host viral variants.

ABSTRACT:

Background

Hepatitis C (HCV) and many other RNA viruses exist as rapidly mutating quasi-species populations in a single infected host. High throughput characterization of full genome, within-host variants is still not possible despite advances in next generation sequencing. This limitation constrains viral genomic studies that depend on accurate identification of hemi-genome or whole genome, within-host variants, especially those occurring at low frequencies. With the advent of third generation long read sequencing technologies, including Oxford Nanopore Technology (ONT) and PacBio platforms, this problem is potentially surmountable. ONT is particularly attractive in this regard due to the portable nature of the MinION sequencer, which makes real-time sequencing in remote and resou

SUBMITTER: Riaz N 

PROVIDER: S-EPMC7923462 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature