Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum.

ABSTRACT:

Background

Sugar content is critically important in determining sugar crop productivity. However, improvement in sugar content has been stagnant among sugar crops for decades. Sorghum, especially sweet sorghum with high biomass, shown great potential for biofuel, has lower sugar content than sugarcane. To enhance sugar content, the sucrose isomerase (SI) gene, driven by stem-specific promoters (A2 or LSG) with a vacuole-targetted signal peptide, was transformed into the sorghum inbred line (T×430).

Results

The study demonstrated that transgenic lines of grain sorghum, containing 50-60% isomaltulose, accumulated up to eightfold (1000 mM) more total sugar than the control T×430 did (118 mM) in stalks of T₀ generation. Subsequently, the elite engineered lines (A5, an

SUBMITTER: Liu G 

PROVIDER: S-EPMC7923521 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature