METTL3-mediated m⁶A modification regulates cell cycle progression of dental pulp stem cells.

ABSTRACT:

Background

Dental pulp stem cells (DPSCs) are a promising cell source in endodontic regeneration and tissue engineering with limited self-renewal and pluripotency capacity. N⁶-methyladenosine (m⁶A) is the most prevalent, reversible internal modification in RNAs associated with stem cell fate determination. In this study, we aim to explore the biological effect of m⁶A methylation in DPSCs.

Methods

m⁶A immunoprecipitation with deep sequencing (m⁶A RIP-seq) demonstrated the features of m⁶A modifications in DPSC transcriptome. Lentiviral vectors were constructed to knockdown or overexpress methyltransferase like 3 (METTL3). Cell morphology, viability, senescence, and apoptosis were analyzed by β-galactosidase, TUN

SUBMITTER: Luo H 

PROVIDER: S-EPMC7923612 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature