A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2.

ABSTRACT: The SARS-CoV-2 pandemic has caused over 1 million deaths globally, mostly due to acute lung injury and acute respiratory distress syndrome, or direct complications resulting in multiple-organ failures. Little is known about the host tissue immune and cellular responses associated with COVID-19 infection, symptoms, and lethality. To address this, we collected tissues from 11 organs during the clinical autopsy of 17 individuals who succumbed to COVID-19, resulting in a tissue bank of approximately 420 specimens. We generated comprehensive cellular maps capturing COVID-19 biology related to patients' demise through single-cell and single-nucleus RNA-Seq of lung, kidney, liver and heart tissues, and further contextualized our findings through spatial RNA profiling of distinct lung regions. We

SUBMITTER: Delorey TM 

PROVIDER: S-EPMC7924267 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature