Novel Three-Finger Neurotoxins from Naja melanoleuca Cobra Venom Interact with GABA_A and Nicotinic Acetylcholine Receptors.

ABSTRACT: Cobra venoms contain three-finger toxins (TFT) including α-neurotoxins efficiently binding nicotinic acetylcholine receptors (nAChRs). As shown recently, several TFTs block GABA_A receptors (GABA_ARs) with different efficacy, an important role of the TFTs central loop in binding to these receptors being demonstrated. We supposed that the positive charge (Arg36) in this loop of α-cobratoxin may explain its high affinity to GABA_AR and here studied α-neurotoxins from African cobra N. melanoleuca venom for their ability to interact with GABAARs and nAChRs. Three α-neurotoxins, close homologues of the known N. melanoleuca long neurotoxins 1 and 2, were isolated and sequenced. Their analysis on Torpedocalifornica and α7 nAChRs, as well a

SUBMITTER: Son L 

PROVIDER: S-EPMC7924340 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature