VpsR Directly Activates Transcription of Multiple Biofilm Genes in Vibrio cholerae.

ABSTRACT: Vibrio cholerae biofilm biogenesis, which is important for survival, dissemination, and persistence, requires multiple genes in the Vibrio polysaccharides (vps) operons I and II as well as the cluster of ribomatrix (rbm) genes. Transcriptional control of these genes is a complex process that requires several activators/repressors and the ubiquitous signaling molecule, cyclic di-GMP (c-di-GMP). Previously, we demonstrated that VpsR directly activates RNA polymerase containing σ⁷⁰ (σ⁷⁰-RNAP) at the vpsL promoter (P _vpsL ), which precedes the vps-II operon, in a c-di-GMP-dependent manner by stimulating formation of the transcriptionally active, open complex. Using in vitro transcription, electrophoretic m

SUBMITTER: Hsieh ML 

PROVIDER: S-EPMC7925080 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature