Subcellular localization of EZH2 phosphorylated at T367 stratifies metaplastic breast carcinoma subtypes.

ABSTRACT:

Background

Metaplastic carcinoma is an aggressive, triple-negative breast cancer (TNBC) with differentiation towards squamous, spindle, or mesenchymal cell types. The molecular underpinnings of the histological subtypes are unclear. Our lab discovered a cytoplasmic function of EZH2, a transcriptional repressor, whereby pEZH2 T367 binds to cytoplasmic proteins in TNBC cells and enhances invasion and metastasis. Here, we investigated the expression and subcellular localization of pEZH2 T367 protein in metaplastic carcinomas.

Methods

Thirty-five metaplastic carcinomas (17 squamous, 10 mesenchymal, and 8 spindle) were evaluated and immunostained with anti-pEZH2 T367. We analyzed staining intensity (score 1-4), subcellular localization (nuclear/cytoplasmic), and localization with

SUBMITTER: McMullen ER 

PROVIDER: S-EPMC7925348 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature