The combination of mitogenic stimulation and DNA damage induces chondrocyte senescence.

ABSTRACT:

Objective

Cellular senescence is a phenotypic state characterized by stable cell-cycle arrest, enhanced lysosomal activity, and the secretion of inflammatory molecules and matrix degrading enzymes. Senescence has been implicated in osteoarthritis (OA) pathophysiology; however, the mechanisms that drive senescence induction in cartilage and other joint tissues are unknown. While numerous physiological signals are capable of initiating senescence, one emerging theme is that damaged cells convert to senescence in response to sustained mitogenic stimulation. The goal of this study was to develop an in vitro articular cartilage explant model to investigate the mechanisms of senescence induction.

Design

This study utilized healthy cartilage derived from cadaveric equine stifles an

SUBMITTER: Copp ME 

PROVIDER: S-EPMC7925350 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature