Enhanced nuclear-spin hyperpolarization of amino acids and proteins via reductive radical quenchers.

ABSTRACT: Low-concentration photochemically induced dynamic nuclear polarization (LC-photo-CIDNP) has recently emerged as an effective tool for the hyperpolarization of aromatic amino acids in solution, either in isolation or within proteins. One factor limiting the maximum achievable signal-to-noise ratio in LC-photo-CIDNP is the progressive degradation of the target molecule and photosensitizer upon long-term optical irradiation. Fortunately, this effect does not cause spectral distortions but leads to a progressively smaller signal buildup upon long-term data-collection (e.g. 500 nM tryptophan on a 600 MHz spectrometer after ca. 200 scans). Given that it is generally desirable to minimize the extent of photodamage, we report that low-μM amounts of the reductive radical quenchers vitamin C (VC, i.

SUBMITTER: Yang H 

PROVIDER: S-EPMC7925436 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature