Formation of contractile 3D bovine muscle tissue for construction of millimetre-thick cultured steak.

ABSTRACT: Owing to the increase in the global demand of meat, cultured meat technology is being developed to circumvent a shortage of meat in the future. However, methods for construction of millimetre-thick bovine muscle tissues with highly aligned myotubes have not yet been established. Here, we propose a culture method for constructing 3D-cultured bovine muscle tissue containing myotubes aligned along its long-axial direction, which contracted in response to electrical stimulation. First, we optimised the composition of biomaterials used in the construction and the electrical stimulation applied to the tissue during culture. Subsequently, we fabricated millimetre-thick bovine muscle tissues containing highly aligned myotubes by accumulating bovine myoblast-laden hydrogel modules. The microbial co

SUBMITTER: Furuhashi M 

PROVIDER: S-EPMC7925560 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature