A CCHFV DNA vaccine protects against heterologous challenge and establishes GP38 as immunorelevant in mice.

ABSTRACT: Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus that causes severe hemorrhagic fever disease in humans. Currently, no licensed CCHF vaccines exist, and the protective epitopes remain unclear. Previously, we tested a DNA vaccine expressing the M-segment glycoprotein precursor gene of the laboratory CCHFV strain IbAr 10200 (CCHFV-M₁₀₂₀₀). CCHFV-M₁₀₂₀₀ provided >60% protection against homologous CCHFV-IbAr 10200 challenge in mice. Here, we report that increasing the dose of CCHFV-M₁₀₂₀₀ provides complete protection from homologous CCHFV challenge in mice, and significant (80%) protection from challenge with the clinically relevant heterologous strain CCHFV-Afg09-2990. We also report complete protection from CCHFV-Afg09-2990 challenge follow

SUBMITTER: Suschak JJ 

PROVIDER: S-EPMC7925670 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature