An Efficient, Counter-Selection-Based Method for Prophage Curing in Pseudomonas aeruginosa Strains.

ABSTRACT: Prophages are bacteriophages in the lysogenic state, where the viral genome is inserted within the bacterial chromosome. They contribute to strain genetic variability and can influence bacterial phenotypes. Prophages are highly abundant among the strains of the opportunistic pathogen Pseudomonas aeruginosa and were shown to confer specific traits that can promote strain pathogenicity. The main difficulty of studying those regions is the lack of a simple prophage-curing method for P. aeruginosa strains. In this study, we developed a novel, targeted-curing approach for prophages in P. aeruginosa. In the first step, we tagged the prophage for curing with an ampicillin resistance cassette (ampR) and further used this strain for the sacB counter-selection mark

SUBMITTER: Shmidov E 

PROVIDER: S-EPMC7926659 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature