Biofunctionalization of Water-Soluble poly(Phenylene Ethynylene)s.

ABSTRACT: We report the use of amide coupling chemistry to covalently link five different biofunctional groups onto an anionic water-soluble poly(phenylene ethynylene) (PPE) polymer. Two of the biofunctionalized PPEs are used in prototype applications, including pH sensing and flow cytometry labeling. The PPE is functionalized with carboxylate (R-CO₂^-) and sulfonate (R-SO₃^-) ionic groups. By using an activated ester, the amine-functionalized groups are covalently linked to the PPE polymer via amide linkages. The reaction chemistry is optimized using biotin-ethylene diamine, making it possible to control the loading of the biotin functionality on the PPE chains. Using the optimized approach, a family of five PPEs were prepared that contain biotin, rhodamine

SUBMITTER: Sun H 

PROVIDER: S-EPMC7927151 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature