LIM and SH3 protein 1 localizes to the leading edge of protruding lamellipodia and regulates axon development.

ABSTRACT: The actin cytoskeleton drives cell motility and is essential for neuronal development and function. LIM and SH3 protein 1 (LASP1) is a unique actin-binding protein that is expressed in a wide range of cells including neurons, but its roles in cellular motility and neuronal development are not well understood. We report that LASP1 is expressed in rat hippocampus early in development, and this expression is maintained through adulthood. High-resolution imaging reveals that LASP1 is selectively concentrated at the leading edge of lamellipodia in migrating cells and axonal growth cones. This local enrichment of LASP1 is dynamically associated with the protrusive activity of lamellipodia, depends on the barbed ends of actin filaments, and requires both the LIM domain and the nebulin repeats of

SUBMITTER: Pollitt SL 

PROVIDER: S-EPMC7927181 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature