The angiogenic potential of CD271+ human adipose tissue-derived mesenchymal stem cells.

ABSTRACT:

Background

Autologous fat grafting is often a crucial aspect of reconstructive and aesthetic surgeries, yet poor graft retention is a major issue with this technique. Enriching fat grafts with adipose tissue-derived mesenchymal stem cells (AD-MSCs) improves graft survival-however, AD-MSCs represent a heterogeneous population. Selection of subpopulations of AD-MSCs would allow the targeting of specific AD-MSCs that may benefit fat graft survival more than the general AD-MSC population.

Methods

Human AD-MSCs were selected for the surface marker CD271 using magnetic-activated cell sorting and compared to the CD271 negative phenotype.  These subpopulations were analysed for gene expression using Real-Time qPCR and RNA sequencing; surface marker characteristics using immunostaini

SUBMITTER: Smith RJP 

PROVIDER: S-EPMC7927269 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature