Optical Detection of CoV-SARS-2 Viral Proteins to Sub-Picomolar Concentrations.

ABSTRACT: The emergence of a new strain of coronavirus in late 2019, SARS-CoV-2, led to a global pandemic in 2020. This may have been preventable if large scale, rapid diagnosis of active cases had been possible, and this has highlighted the need for more effective and efficient ways of detecting and managing viral infections. In this work, we investigate three different optical techniques for quantifying the binding of recombinant SARS-CoV-2 spike protein to surface-immobilized oligonucleotide aptamers. Biolayer interferometry is a relatively cheap, robust, and rapid method that only requires very small sample volumes. However, its detection limit of 250 nM means that it is not sensitive enough to detect antigen proteins at physiologically relevant levels (sub-pM). Surface plasmon resonance is a mo

SUBMITTER: Stanborough T 

PROVIDER: S-EPMC7927290 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature