Comparison of Proteomic Responses as Global Approach to Antibiotic Mechanism of Action Elucidation.

ABSTRACT: New antibiotics are urgently needed to address the mounting resistance challenge. In early drug discovery, one of the bottlenecks is the elucidation of targets and mechanisms. To accelerate antibiotic research, we provide a proteomic approach for the rapid classification of compounds into those with precedented and unprecedented modes of action. We established a proteomic response library of Bacillus subtilis covering 91 antibiotics and comparator compounds, and a mathematical approach was developed to aid data analysis. Comparison of proteomic responses (CoPR) allows the rapid identification of antibiotics with dual mechanisms of action as shown for atypical tetracyclines. It also aids in generating hypotheses on mechanisms of action as presented for salvarsan (arsphenamine) and th

SUBMITTER: Senges CHR 

PROVIDER: S-EPMC7927858 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature