Porous Optically Transparent Cellulose Acetate Scaffolds for Biomimetic Blood-Brain Barrier<i>in vitro</i> Models.

ABSTRACT: In vitro blood-brain barrier (BBB) models represent an efficient platform to conduct high-throughput quantitative investigations on BBB crossing ability of different drugs. Such models provide a closed system where different fundamental variables can be efficaciously tuned and monitored, and issues related to scarce accessibility of animal brains and ethics can be addressed. In this work, we propose the fabrication of cellulose acetate (CA) porous bio-scaffolds by exploiting both vapor-induced phase separation (VIPS) and electrospinning methods. Parameters of fabrication have been tuned in order to obtain porous and transparent scaffolds suitable for optical/confocal microscopy, where endothelial cell monolayers are allowed to growth thus obtaining biomimetic BBB in vitro mod

SUBMITTER: Marino A 

PROVIDER: S-EPMC7928328 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature