Adult mouse dorsal root ganglia neurons form aberrant glutamatergic connections in dissociated cultures.

ABSTRACT: Cultured sensory neurons can exhibit complex activity patterns following stimulation in terms of increased excitability and interconnected responses of multiple neurons. Although these complex activity patterns suggest a network-like configuration, research so far had little interest in synaptic network formation ability of the sensory neurons. To identify interaction profiles of Dorsal Root Ganglia (DRG) neurons and explore their putative connectivity, we developed an in vitro experimental approach. A double transgenic mouse model, expressing genetically encoded calcium indicator (GECI) in their glutamatergic neurons, was produced. Dissociated DRG cultures from adult mice were prepared with a serum-free protocol and no additional growth factors or cytokines were utilized for neuronal sens

SUBMITTER: Bayat FK 

PROVIDER: S-EPMC7928449 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature