A comprehensive protocol for multiplatform metabolomics analysis in patient-derived skin fibroblasts.

ABSTRACT:

Introduction

Patient-derived skin fibroblasts offer a unique translational model to study molecular mechanisms of multiple human diseases. Metabolomics profiling allows to track changes in a broad range of metabolites and interconnected metabolic pathways that could inform on molecular mechanisms involved in disease development and progression, and on the efficacy of therapeutic interventions. Therefore, it is important to establish standardized protocols for metabolomics analysis in human skin fibroblasts for rigorous and reliable metabolic assessment.

Objectives

We aimed to develop an optimized protocol for concurrent measure of the concentration of amino acids, acylcarnitines, and components of the tricarboxylic acid (TCA) cycle in human skin fibroblasts using gas (GC) an

SUBMITTER: Wilkins J 

PROVIDER: S-EPMC7928486 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature