Evolution of combinatorial diversity in trans-acyltransferase polyketide synthase assembly lines across bacteria.

ABSTRACT: Trans-acyltransferase polyketide synthases (trans-AT PKSs) are bacterial multimodular enzymes that biosynthesize diverse pharmaceutically and ecologically important polyketides. A notable feature of this natural product class is the existence of chemical hybrids that combine core moieties from different polyketide structures. To understand the prevalence, biosynthetic basis, and evolutionary patterns of this phenomenon, we developed transPACT, a phylogenomic algorithm to automate global classification of trans-AT PKS modules across bacteria and applied it to 1782 trans-AT PKS gene clusters. These analyses reveal widespread exchange patterns suggesting recombination of extended PKS module series as an important mechanism for metabolic diversification in this natural product class. For three

SUBMITTER: Helfrich EJN 

PROVIDER: S-EPMC7930024 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature