Detection of Cyclospora cayetanensis in produce irrigation and wash water using large-volume sampling techniques.

ABSTRACT: The recent increase of reported cyclosporiasis outbreaks associated with fresh produce has highlighted the need for understanding environmental transmission of Cyclospora cayetanensis in agricultural settings and facilities. Conducting such environmental investigations necessitates robust sample collection and analytical methods to detect C. cayetanensis in water samples. This study evaluated three sample collection methods for recovery of C. cayetanensis oocysts from water samples during seeded recovery experiments. Two filtration-based methods, dead-end ultrafiltration (DEUF) and USEPA Method 1623.1, were evaluated for oocyst recovery from irrigation water. A non-filter-based method, continuous flow centrifugation (CFC), was evaluated separately for recovery from cre

SUBMITTER: Kahler AM 

PROVIDER: S-EPMC7930117 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature