Peripheral blood <i>BRCA1</i> methylation profiling to predict familial ovarian cancer.

ABSTRACT:

Objective

Familial cancer appears at a young age and its incidence is increasing. About 12% of familial ovarian cancer cases are associated with BRCA1/2 mutations (BRCAm). In this study, we investigated BRCA1 methylation may predict ovarian cancer in those with a family history of cancer (FHC) but without BRCA1/2 mutations (BRCAwt).

Methods

Using peripheral blood DNA from 55 subjects without a history of cancer [cancer(-)] and 52 ovarian cancer patients, we examined BRCA1 promoter methylation through bisulfite sequencing of the promoter and expressed the results as the cumulative methylation index. Then, we evaluated the BRCA1 promoter methylation according to BRCA1/2 germline mutations.

Results

BRCA1 methylation was mo

SUBMITTER: Jung Y 

PROVIDER: S-EPMC7930454 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature