Drug target discovery by magnetic nanoparticles coupled mass spectrometry.

ABSTRACT: Drug target discovery is the basis of drug screening. It elucidates the cause of disease and the mechanism of drug action, which is the essential of drug innovation. Target discovery performed in biological systems is complicated as proteins are in low abundance and endogenous compounds may interfere with drug binding. Therefore, methods to track drug-target interactions in biological matrices are urgently required. In this work, a Fe₃O₄ nanoparticle-based approach was developed for drug-target screening in biofluids. A known ligand-protein complex was selected as a principle-to-proof example to validate the feasibility. After incubation in cell lysates, ligand-modified Fe₃O₄ nanoparticles bound to the target protein and formed complexes that wer

SUBMITTER: Xia D 

PROVIDER: S-EPMC7930636 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature