Platelet-derived extracellular vesicles are increased in sera of Alzheimer's disease patients, as revealed by Tim4-based assays.

ABSTRACT: Alzheimer's disease (AD) is the most common form of dementia, characterized by the accumulation of β-amyloid plaques and the formation of neurofibrillary tangles. Extracellular vesicles (EVs) are small vesicles surrounded by a lipid bilayer membrane, which may be involved in the progression of AD. Glycans are essential building blocks of EVs, and we hypothesized that EV glycans may reflect pathological conditions of various diseases. Here, we performed glycan profiling of EVs prepared from sera of three AD patients (APs) compared to three healthy donors (HDs) using lectin microarray. Distinct glycan profiles were observed. Mannose-binding lectins exhibited significantly higher signals for AP-derived EVs than HD-derived EVs. Lectin blotting using mannose-binding lectin (rPALa) showed a sing

SUBMITTER: Odaka H 

PROVIDER: S-EPMC7931225 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature