Co-purification of nitrate reductase 1 with components of the cytochrome bcc-aa₃ oxidase supercomplex from spores of Streptomyces coelicolor A3(2).

ABSTRACT: In order to reduce nitrate in vivo, the spore-specific respiratory nitrate reductase, Nar1, of Streptomyces coelicolor relies on an active cytochrome bcc-aa₃ oxidase supercomplex (bcc-aa₃ supercomplex). This suggests that membrane-associated Nar1, comprising NarG1, NarH1, and NarI1 subunits, might not act as a classical menaquinol oxidase but could either receive electrons from the bcc-aa₃ supercomplex, or require the supercomplex to stabilize the reductase in the membrane to allow it to function. To address the biochemical basis for this dependence on the bcc-aa₃ supercomplex, we purified two different Strep-tagged variants of Nar1 and enriched the native enzyme complex from spore extracts using different chromatographic and electrophoretic proc

SUBMITTER: Falke D 

PROVIDER: S-EPMC7931247 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature