TMEFF2 promoter hypermethylation is an unfavorable prognostic marker in gliomas.

ABSTRACT:

Background

Transmembrane protein with EGF-like and two follistatin-like domains 2 (TMEFF2) is a transmembrane protein in the tomoregulin family. Little research has been performed to determine whether TMEFF2 methylation is a prognostic marker in adult diffuse gliomas.

Methods

In this study, we investigated TMEFF2 expression in surgical glioma tissue samples. In addition, we conducted bisulfite amplicon sequencing (BSAS) and methylation-specific PCR (MSP) to evaluate TMEFF2 methylation in glioblastoma (GBM) cells. Subsequently, we investigated the biological function of TMEFF2 in GBM cells. Moreover, we explored the prognostic significance of TMEFF2 in gliomas by analysing a cohort dataset from TCGA.

Results

Immunohistochemistry analysis of 75 paired glioma tumour and

SUBMITTER: Xie S 

PROVIDER: S-EPMC7931334 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature