Improved node culture methods for rapid vegetative propagation of switchgrass (Panicum virgatum L.).

ABSTRACT:

Background

Switchgrass (Panicum virgatum L.) is an important bioenergy and forage crop. The outcrossing nature of switchgrass makes it infeasible to maintain a genotype through sexual propagation. Current asexual propagation protocols in switchgrass have various limitations. An easy and highly-efficient vegetative propagation method is needed to propagate large natural collections of switchgrass genotypes for genome-wide association studies (GWAS).

Results

Micropropagation by node culture was found to be a rapid method for vegetative propagation of switchgrass. Bacterial and fungal contamination during node culture is a major cause for cultural failure. Adding the biocide, Plant Preservative Mixture (PPM, 0.2%), and the fungicide, Benomyl (5 mg/l), in the incubation solution

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7931530 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature