Molecular Approach to Diagnosis of Cardiovascular Implantable Electronic Device Infection.

ABSTRACT:

Background

Sonicate fluid (SF), a solution derived from vortexing and sonication of explanted cardiovascular implantable electronic devices (CIEDs), is a higher-yield specimen compared with swabs or tissues for culture-based detection of microorganisms associated with CIED infection. Despite this, SF culture fails to identify a causative organism in ~50% of cases. We aimed to evaluate the diagnostic performance of 16S ribosomal RNA gene (rRNA) polymerase chain reaction (PCR)/sequencing of SF and compare it with that of SF culture.

Methods

We identified 322 SF specimens from extracted CIEDs and reviewed clinical data for each patient. Subjects were classified as having or not having CIED infection. Cases were subcategorized as culture negative if no significant growth was rep

SUBMITTER: Esquer Garrigos Z 

PROVIDER: S-EPMC7931840 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature