CreLite: An optogenetically controlled Cre/loxP system using red light.

ABSTRACT:

Background

Precise manipulation of gene expression with temporal and spatial control is essential for functional analysis and determining cell lineage relationships in complex biological systems. The cyclic recombinase (Cre)-loxP system is commonly used for gene manipulation at desired times and places. However, specificity is dependent on the availability of tissue- or cell-specific regulatory elements used in combination with Cre. Here, we present CreLite, an optogenetically controlled Cre system using red light in developing zebrafish embryos.

Results

Cre activity is disabled by splitting Cre and fusing with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6. Upon red light illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together i

SUBMITTER: Yen ST 

PROVIDER: S-EPMC7931845 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature