Simplifying Glycan Profiling through a High-Throughput Micropermethylation Strategy.

ABSTRACT: Glycoproteins play key roles in various molecular and cellular functions and are among the most difficult to analyze biomolecules on account of their microheterogeneity, non-template-driven synthesis, and low abundances. The stability, serum half-life, immunogenicity, and biological activity of therapeutic glycoproteins, including antibodies, vaccines, and biomarkers, are regulated by their glycosylation profile. Thus, there is increasing demand for the qualitative and quantitative characterization and validation of glycosylation on glycoproteins. One of the most important derivatization processes for the structural characterization of released glycans by mass spectrometry (MS) is permethylation. We have recently developed a permethylation strategy in microscale that allows facile permethy

SUBMITTER: Shajahan A 

PROVIDER: S-EPMC7931992 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature