EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis.

ABSTRACT: Erythroblastic islands are a specialized niche that contain a central macrophage surrounded by erythroid cells at various stages of maturation. However, identifying the precise genetic and transcriptional control mechanisms in the island macrophage remains difficult due to macrophage heterogeneity. Using unbiased global sequencing and directed genetic approaches focused on early mammalian development, we find that fetal liver macrophages exhibit a unique expression signature that differentiates them from erythroid and adult macrophage cells. The importance of erythroid Krüppel-like factor (EKLF)/KLF1 in this identity is shown by expression analyses in EKLF-/- and in EKLF-marked macrophage cells. Single-cell sequence analysis simplifies heterogeneity and identifies clusters of genes importa

SUBMITTER: Mukherjee K 

PROVIDER: S-EPMC7932694 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature