CD81 marks immature and dedifferentiated pancreatic β-cells.

ABSTRACT:

Objective

Islets of Langerhans contain heterogeneous populations of insulin-producing β-cells. Surface markers and respective antibodies for isolation, tracking, and analysis are urgently needed to study β-cell heterogeneity and explore the mechanisms to harness the regenerative potential of immature β-cells.

Methods

We performed single-cell mRNA profiling of early postnatal mouse islets and re-analyzed several single-cell mRNA sequencing datasets from mouse and human pancreas and islets. We used mouse primary islets, iPSC-derived endocrine cells, Min6 insulinoma, and human EndoC-βH1 β-cell lines and performed FAC sorting, Western blotting, and imaging to support and complement the findings from the data analyses.

Results

We found that all endocrine cell types express

SUBMITTER: Salinno C 

PROVIDER: S-EPMC7932895 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature