Immobilization Strategies for Enhancing Sensitivity of Electrochemical Aptamer-Based Sensors.

ABSTRACT: Electrochemical aptamer-based (E-AB) sensors are a versatile sensing platform that can achieve rapid and robust target detection in complex matrices. However, the limited sensitivity of these sensors has impeded their translation from proof-of-concept to commercial products. Surface-bound aptamers must be sufficiently spaced to bind targets and subsequently fold for signal transduction. We hypothesized that electrodes fabricated using conventional methods result in sensing surfaces where only a fraction of aptamers are appropriately spaced to actively respond to the target. As an alternative, we presented a novel aptamer immobilization approach that favors sufficient spacing between aptamers at the microscale to achieve optimal target binding, folding, and signal transduction. We first dem

SUBMITTER: Liu Y 

PROVIDER: S-EPMC7933091 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature