Divalent Cation Dependence Enhances Dopamine Aptamer Biosensing.

ABSTRACT: Oligonucleotide receptors (aptamers), which change conformation upon target recognition, enable electronic biosensing under high ionic-strength conditions when coupled to field-effect transistors (FETs). Because highly negatively charged aptamer backbones are influenced by ion content and concentration, biosensor performance and target sensitivities were evaluated under application conditions. For a recently identified dopamine aptamer, physiological concentrations of Mg²⁺ and Ca²⁺ in artificial cerebrospinal fluid produced marked potentiation of dopamine FET-sensor responses. By comparison, divalent cation-associated signal amplification was not observed for FET sensors functionalized with a recently identified serotonin aptamer or a previously reported dopamine apta

SUBMITTER: Nakatsuka N 

PROVIDER: S-EPMC7933093 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature