Long Non-Coding RNA RMRP Contributes to Sepsis-Induced Acute Kidney Injury.

ABSTRACT:

Purpose

This study aimed to explore the role of the long non-coding RNA (lncRNA) RNA component of mitochondrial RNAase P (RMRP) in sepsis-induced acute kidney injury (AKI).

Materials and methods

Venous blood was collected from septic patients and healthy people. C57BL/6 mice who underwent cecal ligation and puncture (CLP) were used as in vivo models of septic AKI. Lipopolysaccharide (LPS)-induced HK-2 cells were employed as in vitro models of AKI. Flow cytometry analysis was conducted to detect cell apoptosis. Enzyme-linked immunosorbent assay and Western blot assays were used to detect levels of pro-inflammatory cytokines.

Results

RMRP was upregulated in sera from patients with AKI and in LPS-induced cells. Knockdown of RMRP inhibited cell apoptosis and reduced produ

SUBMITTER: Zhang X 

PROVIDER: S-EPMC7934096 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature