Bmi-1 alleviates adventitial fibroblast senescence by eliminating ROS in pulmonary hypertension.

ABSTRACT:

Objectives

Pulmonary hypertension (PH) is a life-threatening progressive disease with high mortality in the elderly. However, the pathogenesis of PH has not been fully understood and there is no effective therapy to reverse the disease process. This study aims to determine whether cellular senescence is involved in the development of PH.

Methods

The rat PH model was established by intraperitoneal injection of monocrotaline and evaluated by pulmonary arteriole wall thickness and right ventricular hypertrophy index. Human lung fibroblasts (HLFs) were treated with CoCl₂ or hypoxia to induce cellular senescence in vitro. SA-β-gal staining and the changes of senescent markers were used to examine cellular senescence. The molecular mechanism of cellular senescence was f

SUBMITTER: Li K 

PROVIDER: S-EPMC7934412 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature