Airborne particulate matter (PM_2.5) triggers ocular hypertension and glaucoma through pyroptosis.

ABSTRACT:

Background

Particulate matter (PM) is strongly linked to human health and has detrimental effects on the eye. Studies have, however, focused on the ocular surface, with limited research on the impact of PM_2.5 on intraocular pressure (IOP).

Methods

To investigate the impact of PM_2.5 on IOP and the associated mechanism, C57BL/6 mouse eyes were topically exposed to a PM_2.5 suspension for 3 months, and human trabecular meshwork (HTM) cells were subjected to various PM_2.5 concentrations in vitro. Cell viability, NLRP3/caspase-1, IL-1β, and GSDMD expression, reactive oxygen species (ROS) production and cell contractility were measured by western blot, ELISA, cell counting kit-8, ROS assay kit or a cell contractility assay. ROS scavenger

SUBMITTER: Li L 

PROVIDER: S-EPMC7934500 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature