De novo genome assembly of two tomato ancestors, Solanum pimpinellifolium and Solanum  lycopersicum var. cerasiforme, by long-read sequencing.

ABSTRACT: The ancestral tomato species are known to possess genes that are valuable for improving traits in breeding. Here, we aimed to construct high-quality de novo genome assemblies of Solanum pimpinellifolium 'LA1670' and S. lycopersicum var. cerasiforme 'LA1673', originating from Peru. The Pacific Biosciences (PacBio) long-read sequences with 110× and 104× coverages were assembled and polished to generate 244 and 202 contigs spanning 808.8 Mbp for 'LA1670' and 804.5 Mbp for 'LA1673', respectively. After chromosome-level scaffolding with reference guiding, 14 scaffold sequences corresponding to 12 tomato chromosomes and 2 unassigned sequences were constructed. High-quality genome assemblies were confirmed using the Benchmarking Universal Single-Copy Orthologs and long terminal repeat assembly in

SUBMITTER: Takei H 

PROVIDER: S-EPMC7934570 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature