Dataset Information

Diagnostic accuracy comparison of three fully automated chemiluminescent immunoassay platforms for the detection of SARS-CoV-2 antibodies.

ABSTRACT:

Background

Serological test is an essential surveillance tool to track down the extensiveness of SARS-CoV-2 transmission and subsequently to move out from the enforced lockdown stage.

Objective

The study measures the diagnostic accuracy of three popular chemiluminescent immunoassay (CLIA) based automated platforms for the detection of anti-SARS-CoV-2 antibodies and compares their agreements.

Study design

Serum samples of 594 COVID-19 positive patients and 100 samples from pre-COVID cases were tested by three CLIA based automated platforms: Abbott architect i2000SR, Roche cobas e411 and Yhlo iFlash 1800 and their diagnostic accuracy were compared by the area under the curves (AUC) value obtained from receiver operator characteristic (ROC) curves. Cohen's kappa statistic and McNemar's test were used to interpret the agreement between the platforms.

Results

All three platforms showed high specificity as claimed by the manufacturer. Sensitivity was calculated as 64.48 % (58.67-70.3) for Abbott, 80.48 % (76.62-84.34) for Roche and 76.94 % (72.65-81.23) for Yhlo. AUC was maximum for Roche (0.929). The Cohen's kappa value was determined in between 0.69-0.89 as the inter-rater agreements.

Conclusion

The overall statistical analysis demonstrated cobas e411 as the diagnostically most accurate platform among the three.

SUBMITTER: Parai D

PROVIDER: S-EPMC7934617 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Publications

Diagnostic accuracy comparison of three fully automated chemiluminescent immunoassay platforms for the detection of SARS-CoV-2 antibodies.

Parai Debaprasad D Dash Girish Chandra GC Choudhary Hari Ram HR Peter Annalisha A Rout Usha Kiran UK Nanda Rashmi Ranjan RR Kshatri Jaya Singh JS Kanungo Srikanta S Pati Sanghamitra S Bhattacharya Debdutta D

Journal of virological methods 20210305

<h4>Background</h4>Serological test is an essential surveillance tool to track down the extensiveness of SARS-CoV-2 transmission and subsequently to move out from the enforced lockdown stage.<h4>Objective</h4>The study measures the diagnostic accuracy of three popular chemiluminescent immunoassay (CLIA) based automated platforms for the detection of anti-SARS-CoV-2 antibodies and compares their agreements.<h4>Study design</h4>Serum samples of 594 COVID-19 positive patients and 100 samples from p ...[more]

PMID: 33684402

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Dataset Information

Diagnostic accuracy comparison of three fully automated chemiluminescent immunoassay platforms for the detection of SARS-CoV-2 antibodies.

Background

Objective

Study design

Results

Conclusion

Publications

Diagnostic accuracy comparison of three fully automated chemiluminescent immunoassay platforms for the detection of SARS-CoV-2 antibodies.

Similar Datasets

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