Comparison of porcine corneal decellularization methods and importance of preserving corneal limbus through decellularization.

ABSTRACT:

Background

The aim of this study is to compare the three previously applied, conventional porcine corneal decellularization methods and to demonstrate the importance of preserving the corneal limbus through decellularization.

Methods

Fresh, wild-type (with or without) limbus porcine corneas were decellularized using three different methods, including (i) sodium dodecyl sulfate (SDS), (ii) hypertonic saline (HS), and (iii) N2 gas (NG). Post-treatment evaluation was carried out using histological, residual nuclear material, and ultrastructural analyses. Glycerol was used to help reduce the adverse effects of decellularization. The corneas were preserved for two weeks in cornea storage medium.

Results

All three decellularization methods reduced the number of keratocytes

SUBMITTER: Isidan A 

PROVIDER: S-EPMC7935270 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature