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How varying parameters impact insecticide resistance bioassay: An example on the worldwide invasive pest Drosophila suzukii.


ABSTRACT: Monitoring pesticide resistance is essential for effective and sustainable agricultural practices. Bioassays are the basis for pesticide-resistance testing, but devising a reliable and reproducible method can be challenging because these tests are carried out on living organisms. Here, we investigated five critical parameters and how they affected the evaluation of resistance to the organophosphate phosmet or the pyrethroid lambda-cyhalothrin using a tarsal-contact protocol on Drosophila suzukii, a worldwide invasive pest. Three of the parameters were related to insect biology: (i) sex, (ii) age of the imago (adult stage) and (iii) genetic diversity of the tested population. The two remaining parameters were linked to the experimental setup: (iv) the number of individuals tested per dose a

SUBMITTER: Blouquy L 

PROVIDER: S-EPMC7935283 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature