Profiling of the Human Natural Killer Cell Receptor-Ligand Repertoire.

ABSTRACT: Natural killer (NK) cells are among the first responders to viral infections. The ability of NK cells to rapidly recognize and kill virally infected cells is regulated by their expression of germline-encoded inhibitory and activating receptors. The engagement of these receptors by their cognate ligands on target cells determines whether the intercellular interaction will result in NK cell killing. This protocol details the design and optimization of two complementary mass cytometry (CyTOF) panels. One panel was designed to phenotype NK cells based on receptor expression. The other panel was designed to interrogate expression of known ligands for NK cell receptors on several immune cell subsets. Together, these two panels allow for the profiling of the human NK cell receptor-ligand repertoi

SUBMITTER: Vendrame E 

PROVIDER: S-EPMC7935321 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature