Clostridioides difficile Whole-genome Sequencing Differentiates Relapse With the Same Strain From Reinfection With a New Strain.

ABSTRACT:

Background

Current approaches in tracking Clostridioides difficile infection (CDI) and individualizing patient management are incompletely defined.

Methods

We recruited 468 subjects with CDI at Mayo Clinic Rochester between May and December 2016 and performed whole-genome sequencing (WGS) on C. difficile isolates from 397. WGS was also performed on isolates from a subset of the subjects at the time of a recurrence of infection. The sequence data were analyzed by determining core genome multilocus sequence type (cgMLST), with isolates grouped by allelic differences and the predicted ribotype.

Results

There were no correlations between C. difficile isolates based either on cgMLST or ribotype groupings and CDI outcome. An epidemiologic assessment of hospitalized subjects

SUBMITTER: Cho J 

PROVIDER: S-EPMC7935391 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature