Retrograde ERK activation waves drive base-to-apex multicellular flow in murine cochlear duct morphogenesis.

ABSTRACT: A notable example of spiral architecture in organs is the mammalian cochlear duct, where the morphology is critical for hearing function. Genetic studies have revealed necessary signaling molecules, but it remains unclear how cellular dynamics generate elongating, bending, and coiling of the cochlear duct. Here, we show that extracellular signal-regulated kinase (ERK) activation waves control collective cell migration during the murine cochlear duct development using deep tissue live-cell imaging, Förster resonance energy transfer (FRET)-based quantitation, and mathematical modeling. Long-term FRET imaging reveals that helical ERK activation propagates from the apex duct tip concomitant with the reverse multicellular flow on the lateral side of the developing cochlear duct, resulting in ad

SUBMITTER: Ishii M 

PROVIDER: S-EPMC7935486 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature