Whole-organ analysis of TGF-β-mediated remodelling of the tumour microenvironment by tissue clearing.

ABSTRACT: Tissue clearing is one of the most powerful strategies for a comprehensive analysis of disease progression. Here, we established an integrated pipeline that combines tissue clearing, 3D imaging, and machine learning and applied to a mouse tumour model of experimental lung metastasis using human lung adenocarcinoma A549 cells. This pipeline provided the spatial information of the tumour microenvironment. We further explored the role of transforming growth factor-β (TGF-β) in cancer metastasis. TGF-β-stimulated cancer cells enhanced metastatic colonization of unstimulated-cancer cells in vivo when both cells were mixed. RNA-sequencing analysis showed that expression of the genes related to coagulation and inflammation were up-regulated in TGF-β-stimulated cancer cells. Further, whole-organ a

SUBMITTER: Kubota SI 

PROVIDER: S-EPMC7935961 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature